sigmaplot scientific graphing software windows version 8.0 Search Results


sigmaplot 8.0  (SPSS Inc)
90
SPSS Inc sigmaplot 8.0
FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.
Sigmaplot 8.0, supplied by SPSS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sigmaplot (version 8.0  (SPSS Inc)
90
SPSS Inc sigmaplot (version 8.0
FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.
Sigmaplot (Version 8.0, supplied by SPSS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sigmaplot (version 8.0 - by Bioz Stars, 2026-05
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sigmaplot  (SPSS Inc)
90
SPSS Inc sigmaplot
FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.
Sigmaplot, supplied by SPSS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sigmaplot/product/SPSS Inc
Average 90 stars, based on 1 article reviews
sigmaplot - by Bioz Stars, 2026-05
90/100 stars
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sigmaplot 8.0  (HULINKS Inc)
90
HULINKS Inc sigmaplot 8.0
FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.
Sigmaplot 8.0, supplied by HULINKS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sigmaplot 8.0/product/HULINKS Inc
Average 90 stars, based on 1 article reviews
sigmaplot 8.0 - by Bioz Stars, 2026-05
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sigmaplot 8.0  (TIBCO)
90
TIBCO sigmaplot 8.0
FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.
Sigmaplot 8.0, supplied by TIBCO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sigmaplot 8.0/product/TIBCO
Average 90 stars, based on 1 article reviews
sigmaplot 8.0 - by Bioz Stars, 2026-05
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sigmaplot 8.0  (Jandel Engineering)
90
Jandel Engineering sigmaplot 8.0
FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.
Sigmaplot 8.0, supplied by Jandel Engineering, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sigmaplot 8.0/product/Jandel Engineering
Average 90 stars, based on 1 article reviews
sigmaplot 8.0 - by Bioz Stars, 2026-05
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sigmastat v2.03  (SPSS Inc)
90
SPSS Inc sigmastat v2.03
FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.
Sigmastat V2.03, supplied by SPSS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sigmastat v2.03/product/SPSS Inc
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sigmastat v2.03 - by Bioz Stars, 2026-05
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sigmastat 2.0  (SPSS Inc)
90
SPSS Inc sigmastat 2.0
FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.
Sigmastat 2.0, supplied by SPSS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sigmastat 2.0/product/SPSS Inc
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sigmastat 2.0 - by Bioz Stars, 2026-05
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sigmastat 3.0  (Jandel Engineering)
90
Jandel Engineering sigmastat 3.0
FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.
Sigmastat 3.0, supplied by Jandel Engineering, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sigmastat 3.0/product/Jandel Engineering
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sigmastat 3.0 - by Bioz Stars, 2026-05
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origin 8.0  (OriginLab corp)
90
OriginLab corp origin 8.0
FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.
Origin 8.0, supplied by OriginLab corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/origin 8.0/product/OriginLab corp
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origin 8.0 - by Bioz Stars, 2026-05
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sigmastat  (SPSS Inc)
90
SPSS Inc sigmastat
FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.
Sigmastat, supplied by SPSS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sigmastat/product/SPSS Inc
Average 90 stars, based on 1 article reviews
sigmastat - by Bioz Stars, 2026-05
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software origin 8.0  (OriginLab corp)
90
OriginLab corp software origin 8.0
FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.
Software Origin 8.0, supplied by OriginLab corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/software origin 8.0/product/OriginLab corp
Average 90 stars, based on 1 article reviews
software origin 8.0 - by Bioz Stars, 2026-05
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Image Search Results


FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.

Journal:

Article Title: RecR-mediated Modulation of RecF Dimer Specificity for Single- and Double-stranded DNA * S⃞

doi: 10.1074/jbc.M806378200

Figure Lengend Snippet: FRET measurements of ATP-dependent RecF dimerization. A, reactions were carried out as described under “Experimental Procedures.” Signals from FAM and Cy3 labels were measured upon titration of an equimolar mixture of FAM- and Cy3-labeled RecF proteins with ATP or ADP. Changes of FRET signal in arbitrary units were plotted against corresponding nucleotide concentrations. The lanes represent theoretical fit performed with Scientist software and plotted with SigmaPlot 8.0 (SPSS Inc.). Solid, long-dash, medium-dash, short-dash, dotted, and dash-dotted lines with filled symbols correspond to 0.1, 0.2, 0.4, 1, 2, and 4 μm concentrations of RecF, respectively. The solid line with open circles corresponds to titration of 2 μm RecF with ADP. B, a secondary plot of the Bmax values versus the RecF concentrations derived from the FRET measurements shown in A. The curve represents theoretical fitting of data with SigmaPlot.

Article Snippet: The graphs were created with SigmaPlot 8.0 (SPSS Inc.).

Techniques: Titration, Labeling, Software, Derivative Assay

RecF-DNA interaction measured by the fluorescence polarization assay. The labeled DNA substrates (20 nm) were titrated with RecF in the presence of 2 mm nucleotide. RecR was included where indicated at final concentration of 30 μm. Normalized fluorescence polarization signal in arbitrary units was plotted against protein concentrations. The graphs were created with SigmaPlot 8.0 (SPSS Inc.). RecF interactions with ssDNA (S1) are shown in panel A, with dsDNA (D1) in panel B, and with ds/ssDNA (J1) junction in panel C. Dotted lines represent the interaction of the RecF(S279R) mutant with DNA in the presence of ATP; short-dashed lines, a wild-type RecF in the presence of ADP; solid lines, a wild-type RecF in the presence of ATP; long-dashed lines, a wild-type RecF in the presence of ATP and RecR.

Journal:

Article Title: RecR-mediated Modulation of RecF Dimer Specificity for Single- and Double-stranded DNA * S⃞

doi: 10.1074/jbc.M806378200

Figure Lengend Snippet: RecF-DNA interaction measured by the fluorescence polarization assay. The labeled DNA substrates (20 nm) were titrated with RecF in the presence of 2 mm nucleotide. RecR was included where indicated at final concentration of 30 μm. Normalized fluorescence polarization signal in arbitrary units was plotted against protein concentrations. The graphs were created with SigmaPlot 8.0 (SPSS Inc.). RecF interactions with ssDNA (S1) are shown in panel A, with dsDNA (D1) in panel B, and with ds/ssDNA (J1) junction in panel C. Dotted lines represent the interaction of the RecF(S279R) mutant with DNA in the presence of ATP; short-dashed lines, a wild-type RecF in the presence of ADP; solid lines, a wild-type RecF in the presence of ATP; long-dashed lines, a wild-type RecF in the presence of ATP and RecR.

Article Snippet: The graphs were created with SigmaPlot 8.0 (SPSS Inc.).

Techniques: Fluorescence, Labeling, Concentration Assay, Mutagenesis